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non malignant human pancreatic duct epithelial cell line crl  (ATCC)


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    ATCC non malignant human pancreatic duct epithelial cell line crl
    Non Malignant Human Pancreatic Duct Epithelial Cell Line Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pancreatic+duct+epithelial+cell+line/hTERT-HPNE/pm42278260-146-17-29
    Average 96 stars, based on 458 article reviews
    non malignant human pancreatic duct epithelial cell line crl - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: lncRNA DGCR 5/miR-27a-3p/BNIP3 promotes cell apoptosis in pancreatic cancer by regulating the p38 MAPK pathway
    Article Snippet: .. The human PaCa cell lines, SW1990 and PANC-1 [American Type Culture Collection (ATCC)], and the normal human pancreatic duct epithelial cell line, HPDE6-C7 (ATCC), were cultured in standard DMEM containing 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml of penicillin, and 100 μ g/ml of streptomycin (HyClone; GE Healthcare Life Sciences) and maintained at 37°C in a humidified atmosphere with 5% CO 2 . ..

    Article Title: CircATP13A1 (hsa_circ_0000919) promotes cell proliferation and metastasis and inhibits cell apoptosis in pancreatic ductal adenocarcinoma via the miR-186/miR-326/HMGA2 axis: implications for novel therapeutic targets
    Article Snippet: .. The normal human pancreatic duct epithelial cell line (HPDE6c7) and PDAC cancer cell lines including AsPC-1, CFPAC, SW1990, BxPC-3, and Panc-1 were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS, 100 U/ml penicillin, and 100 g/ml streptomycin (Beyotime Institute of Biotechnology) at 37°C. ..

    Article Title: CircATP13A1 (hsa_circ_0000919) promotes cell proliferation and metastasis and inhibits cell apoptosis in pancreatic ductal adenocarcinoma via the miR-186/miR-326/HMGA2 axis: implications for novel therapeutic targets
    Article Snippet: .. Cell lines and culture The normal human pancreatic duct epithelial cell line (HPDE6c7) and PDAC cancer cell lines including AsPC-1, CFPAC, SW1990, BxPC-3, and Panc-1 were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS, 100 U/ml penicillin, and 100 g/ml streptomycin (Beyotime Institute of Biotechnology) at 37°C. ..

    Article Title: Discovery of cadinane sesquiterpenoids as GOT1 inhibitors from Penicillium sp. HZ-5.
    Article Snippet: Fifteen new cadinane sesquiterpenoids, amorphaenes A–O (1–15), along with one known compound, were isolated from an endophytic fungus Penicillium sp. HZ-5 collected from Hypericum wilsonii N. Robson.. Notably, compound 7 was the first example of 11-nor cadinane sesquiterpenoid via the oxidative cleavage between C-11 and C-13.. Their structures were elucidated by extensive spectroscopic analysis, singlecrystal X-ray diffraction and ECD calculation and comparison.

    Article Title: Discovery of GOT1 Inhibitors from a Marine-Derived Aspergillus terreus That Act against Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. Pancreas (SW1990, AsPC-1, PANC-1), colorectal (HCT116), breast (HCC1806, MM231, MM453), and ovarian cancer cells (ES2), along with a nonmalignant human pancreatic duct epithelial cell line (HDPE6C7), were obtained from ATCC, and were cultured in 1640 medium or Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% ( v/v ) fetal bovine serum (FBS), 100 μg/mL streptomycin, and 100 U/mL penicillin. ..

    Modification:

    Article Title: Discovery of cadinane sesquiterpenoids as GOT1 inhibitors from Penicillium sp. HZ-5.
    Article Snippet: Fifteen new cadinane sesquiterpenoids, amorphaenes A–O (1–15), along with one known compound, were isolated from an endophytic fungus Penicillium sp. HZ-5 collected from Hypericum wilsonii N. Robson.. Notably, compound 7 was the first example of 11-nor cadinane sesquiterpenoid via the oxidative cleavage between C-11 and C-13.. Their structures were elucidated by extensive spectroscopic analysis, singlecrystal X-ray diffraction and ECD calculation and comparison.

    Article Title: Discovery of GOT1 Inhibitors from a Marine-Derived Aspergillus terreus That Act against Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. Pancreas (SW1990, AsPC-1, PANC-1), colorectal (HCT116), breast (HCC1806, MM231, MM453), and ovarian cancer cells (ES2), along with a nonmalignant human pancreatic duct epithelial cell line (HDPE6C7), were obtained from ATCC, and were cultured in 1640 medium or Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% ( v/v ) fetal bovine serum (FBS), 100 μg/mL streptomycin, and 100 U/mL penicillin. ..



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    Fig. 2 CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C–E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR anal- ysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal <t>HPDE,</t> parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. *P < 0.05
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    CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C – E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR analysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal <t>HPDE,</t> parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. * P < 0.05
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    Image Search Results


    Effect of Ech alone or in combination with TC-HT on the cell viability. (A) MTT viability assay of PANC-1 cells treated with different concentrations of Ech or in combination with TC-HT treatment. (B) MTT viability assay of H6c7 normal human pancreatic cells treated with 20 μM Ech or in combination with the same TC-HT treatment. Data represent the mean ± standard deviation ( n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test (*** P < 0.001).

    Journal: Annals of Medicine and Surgery

    Article Title: Combination of cycling hyperthermia and echinacoside creates a synergistic curing effect on pancreatic cancer PANC-1 cells

    doi: 10.1097/MS9.0000000000003545

    Figure Lengend Snippet: Effect of Ech alone or in combination with TC-HT on the cell viability. (A) MTT viability assay of PANC-1 cells treated with different concentrations of Ech or in combination with TC-HT treatment. (B) MTT viability assay of H6c7 normal human pancreatic cells treated with 20 μM Ech or in combination with the same TC-HT treatment. Data represent the mean ± standard deviation ( n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test (*** P < 0.001).

    Article Snippet: H6c7 human pancreatic duct epithelial cell line was obtained from Kerafast, Inc. (Absolute Biotech, Boston, MA, USA) and maintained in keratinocyte serum-free medium (Invitrogen; Thermo Fisher Scientific, Inc., Carlsbad, CA, USA) supplemented with human recombinant epidermal growth factor, bovine pituitary extract (Invitrogen; Thermo Fisher Scientific, Inc.), and 1% (v/v) penicillin and streptomycin.

    Techniques: MTT Viability Assay, Standard Deviation

    Fig. 2 CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C–E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR anal- ysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal HPDE, parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. *P < 0.05

    Journal: Discover oncology

    Article Title: Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4.

    doi: 10.1007/s12672-025-01825-8

    Figure Lengend Snippet: Fig. 2 CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C–E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR anal- ysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal HPDE, parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. *P < 0.05

    Article Snippet: :(0123456789) Human pancreatic duct epithelial (HPDE) cell line (Cat#CL-0921, Procell) was cultured in keratinocyte serum-free medium containing epidermal growth factor, bovine pituitary extract (Cat#:17005042, Invitrogen, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Cat#PB180120, Procell) at 37 °C with 5% CO2.

    Techniques: Expressing, Western Blot, Incubation, Quantitative RT-PCR

    CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C – E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR analysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal HPDE, parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. * P < 0.05

    Journal: Discover Oncology

    Article Title: Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4

    doi: 10.1007/s12672-025-01825-8

    Figure Lengend Snippet: CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C – E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR analysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal HPDE, parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. * P < 0.05

    Article Snippet: Human pancreatic duct epithelial (HPDE) cell line (Cat#CL-0921, Procell) was cultured in keratinocyte serum-free medium containing epidermal growth factor, bovine pituitary extract (Cat#:17005042, Invitrogen, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Cat#PB180120, Procell) at 37 °C with 5% CO 2 .

    Techniques: Expressing, Western Blot, Incubation, Quantitative RT-PCR